Table 1 of Ahmad, Mol Vis 2011; 17:1940-1945. Table 1 of Ahmad, Mol Vis 2011; 17:1940-1945.
Table 1. Primers used for PCR amplification and direct sequencing of LCA5 coding exons and adjacent intronic regions.
| Exon | Primer sequence | tm (°C) | cds (bp) | Product size (bp) |
|---|---|---|---|---|
| 3F | TGTGGAGAAAATAGATTGCACAG | 59.28 | ||
| 3R | CCTATAAAACGTAAATCAGCCCAC | 60.14 | 190 | 465 |
| 4F | AGAATAATTCCGTATAAACTATTGGG | 57.42 | ||
| 4R | TTTTCCCAAAATGACTATGATCC | 59.22 | 530 | 996 |
| 5F | TGTACATGAATACTATGCCCAGTC | 58.10 | ||
| 5R | TTATACCAACAAACCTTTTCTAAGTG | 57.26 | 138 | 437 |
| 6F | CAAAAGGAAGCTGAACCAGG | 59.85 | ||
| 6R | CAGCGTCACTTCAGGGG | 58.83 | 97 | 420 |
| 7F | CCAAGCTGAGCAAAACATGC | 61.88 | ||
| 7R | TTAGGTATATCTCCTAAAAGCCAAAG | 56.64 | 143 | 538 |
| 8F | TTCAAGGAGTGATAACTTGTGATTTAC | 59.12 | ||
| 8R | TAAGCCATCCCCTACCACTG | 59.95 | 133 | 437 |
| 9F | TCTTTTCTCACTTGATTTATAATACCC | 57.59 | ||
| 9R | TTGGCAAACTATCTATGTGGTG | 57.72 | 863 | 1167 |