Figure 5. Glial fibrillary acidic protein (GFAP) localization in retinal flat-mounts of alkali injured mice at 14 days post-injury. A: Prominent GFAP expression as observed in this image montage is visible throughout the retina most notably upregulated in astrocytes. B: Enlarged image of activated Muller cells. C: Representative enlarged image of astrocytes showing GFAP staining. For orientation purposes, the optic nerve is marked with a white semi-circular line. The scale bar in the montage image (A) is 215 μm. The scale bars in the enlarged images are 70 μm.