Figure 3 of Paranthan, Mol Vis 2011; 17:1901-1908.


Figure 3. Fourteen days post-injury gliosis in the retina. Mice were subjected to corneal alkali injury with limbal and corneal epithelial cell debridement and treated daily with dimethyl sulfoxide (DMSO, Veh) or 2 mg/kg withaferin A (WFA) for 14 days. A-C: Tissue sections of eyes from uninjured (Cont), injured (veh) and WFA treated mice were stained with antibodies to glial fibrillary acidic protein (GFAP; green). Nuclei were stained with 4′, 6-diamidino-2-phenylindole (DAPI, blue) and fluorescent staining was visualized with a Nikon TE2000 microscope. D: western blot analysis of GFAP expression in soluble extracts of retina samples from uninjured (cont), injured (Veh) and WFA treated mice. Abbreviations: GCL represents ganglion cell layer; INL represents inner nuclear layer; ONL represents outer nuclear layer.