Table 2 of Anshu, Mol Vis 2011; 17:1891-1900.

Table 2. Proteins identified in the albumin-depleted fraction of aqueous humor in patients with glaucoma shunt device using Liquid Chromatography/Mass Spectrometry (LC-MS/MS).



Protein ID

Protein name		 Protein
coverage
(%)		 Number of
unique
sequences		 CV
(%)
normal		 CV
(%)
shunt		 Fold change
in shunt
patients		 p-value
(shunt
versus normal)
Plasma
protein
IPI00026314 Gelsolin 5.12 2 97.38 42.91 4.7 0.000050 Yes
IPI00019580 Plasminogen 21.98 10 66.75 47.85 6.2 0.000090 Yes
IPI00032220 Angiotensinogen 16.49 5 50.43 46.49 4.4 0.000100 Yes
IPI00021854 Apolipoprotein A-II 64 6 71.48 58.76 18.5 0.000200 Yes
IPI00004656 Beta-2-microglobulin 18.49 1 33.81 49.13 5.7 0.000200 Yes
IPI00383937 cDNA FLJ33633 fis, clone BRAMY2022786, highly similar to Homo sapiens dickkopf-3 (DKK-3) mRNA 30.7 4 43.93 45.25 4 0.000200 Yes
IPI00002714 cDNA FLJ52545, highly similar to Dickkopf-related protein 3 18.13 4 43.93 45.25 4 0.000200 Yes
IPI00006114 Pigment epithelium-derived factor 38.28 12 54.02 48.22 4.6 0.000200 Yes
IPI00386812 RIG-like 7–1 23.39 2 35.69 52.57 7.2 0.000200 Yes
IPI00019943 Afamin 13.86 5 53.94 45.3 3.3 0.000300 Yes
IPI00922213 cDNA FLJ53292, highly similar to Homo sapiens fibronectin 1 (FN1), transcript variant 5, mRNA 2.17 1 53.67 53.43 6.2 0.000300 Yes
IPI00021841 Apolipoprotein A-I 54.31 17 49.82 55.18 6.4 0.000400 Yes
IPI00889723 C4A protein 18.43 18 60.51 50.69 4.1 0.000400 No
IPI00019568 Prothrombin (Fragment) 18.81 7 59.4 43.88 2.9 0.000400 Yes
IPI00937598 similar to C4A protein isoform 2 15.9 15 59.85 45.91 3.3 0.000400 No
IPI00017601 Ceruloplasmin 32.68 24 51.22 52.5 4.5 0.000500  
IPI00022488 Hemopexin 53.9 17 73.75 56.72 6.2 0.000500  
IPI00215894 Kininogen-1, Isoform LMW 26.93 8 49.09 47.4 3.2 0.000600  
IPI00789376 KNG1 protein 34.36 7 48.72 47.54 3.1 0.000700  
IPI00163207 N-acetylmuramoyl-L-alanine amidase 4.69 2 64.87 53.66 4.2 0.000700  
IPI00909649 Protein 49.53 3 52.92 61.2 7.2 0.000800  
IPI00893864 Complement factor B 6.45 2 24.5 55.96 4.4 0.000900  
IPI00022420 Retinol-binding protein 4 33.33 4 63.72 53.93 3.9 0.000900  
IPI00022463 Serotransferrin 67.62 50 66.88 50.61 3.4 0.000900  
IPI00032179 Antithrombin-III 43.1 14 62.13 59.34 4.7 0.001000  
IPI00922058 cDNA FLJ59472, highly similar to Tripeptidyl-peptidase 1 7.8 1 28.97 41.42 2.3 0.001000  
IPI00783987 Complement C3 (Fragment) 29.95 34 57.03 54.67 3.6 0.001000  
IPI00892547 Complement component 4A 20.3 20 59.62 59.21 5 0.001000  
IPI00027482 Corticosteroid-binding globulin 10.12 2 45.96 61.64 5.4 0.001000  
IPI00556287 Putative uncharacterized protein 17.99 2 34.29 56.42 4.3 0.001000  
IPI00924948 Putative uncharacterized protein AZGP1 26.87 4 66.28 55.65 4 0.001000  
IPI00643525 Putative uncharacterized protein C4A 21.33 21 58.55 59.2 5.2 0.001000  
IPI00739237 similar to complement component 3 4.87 1 42.7 58.4 4.7 0.001000  
IPI00935601 similar to complement component 4B (Childo blood group), partial 2.85 1 92.62 61.48 6 0.001000  
IPI00298971 Vitronectin 13.6 4 56.8 59.77 5.1 0.001000  
IPI00020091 Alpha-1-acid glycoprotein 2 39.8 8 74.23 60.87 4.2 0.002000  
IPI00022895 Alpha-1B-glycoprotein 35.96 11 64.92 68.72 6.5 0.002000  
IPI00166729 alpha-2-glycoprotein 1, zinc precursor 40.6 9 55.39 51.01 2.9 0.002000  
IPI00478003 Alpha-2-macroglobulin 11.47 11 56.79 54.7 3.3 0.002000  
IPI00218748 Beta-crystallin B2 19.51 4 95.69 65.53 5.7 0.002000  
IPI00019591 cDNA FLJ55673, highly similar to Complement factor B 5.45 4 44.43 58.6 4.2 0.002000  
IPI00032258 Complement C4-A 20.07 20 58 62.3 5 0.002000  
IPI00654875 Complement C4-B 18.18 18 59.41 55.54 3.5 0.002000  
IPI00218192 Inter-alpha-trypsin inhibitor heavy chain H4 7.33 4 66.74 67.97 7.6 0.002000  
IPI00032328 Kininogen-1, Isoform HMW 15.84 7 50.67 51.24 2.9 0.002000  
IPI00384938 Putative uncharacterized protein DKFZp686N02209 29.88 9 68.21 57.76 3.6 0.002000  
IPI00014048 RNase pancreatic 13.46 1 52.72 61.62 4.8 0.002000  
IPI00553177 Alpha-1-antitrypsin 66.27 30 75.69 68.19 5.9 0.003000  
IPI00947307 cDNA FLJ58075, highly similar to Ceruloplasmin 15.75 9 75.54 71.62 7.1 0.003000  
IPI00892604 Complement component C4B (Childo blood group) 2 26.09 27 57.45 64.62 4.5 0.003000  
IPI00013179 Prostaglandin-H2 D-isomerase 32.63 3 59.4 52.56 2.7 0.003000  
IPI00930124 Putative uncharacterized protein DKFZp686C11235 24.1 7 68.59 60.18 3.6 0.003000  
IPI00947496 124 kDa protein 6.46 5 72.67 68.22 4.4 0.004000  
IPI00939333 86 kDa protein 11.13 5 57.33 57.59 3 0.004000  
IPI00477597 Haptoglobin-related protein 20.98 7 48.71 67.78 4.6 0.004000  
IPI00745872 Serum albumin 56.98 31 62.39 65.64 4 0.004000  
IPI00796888 26 kDa protein 7.76 1 28.92 68.46 4.1 0.005000  
IPI00942927 cDNA FLJ57339, highly similar to Complement C3 5.28 3 74.79 75.8 6.2 0.005000  
IPI00796830 13 kDa protein 10.53 1 63.45 55.98 2.6 0.006000  
IPI00304273 Apolipoprotein A-IV 37.37 14 62.49 69.08 4.1 0.006000  
IPI00924859 kininogen-1, Isoform 3 26.6 7 53.65 61.14 3 0.006000  
IPI00555812 Vitamin D-binding protein 42.62 17 62.88 76.48 5.7 0.006000  
IPI00022426 Protein AMBP 17.61 4 78.76 46.73 2.4 0.007000  
IPI00550991 cDNA FLJ35730 fis, clone TESTI2003131, highly similar to ALPHA-1-ANTICHYMOTRYPSIN 43.53 16 51.65 68.56 3.6 0.008000  
IPI00908876 cDNA FLJ50830, highly similar to Serum albumin 7.27 2 126.5 89.61 13 0.009000  
IPI00879709 Complement component 6 precursor 2.65 1 137.14 82.89 6.9 0.009000  
IPI00884251 ENV polyprotein (coat polyprotein) family protein 5.29 1 101.32 85.96 8.3 0.009000  
IPI00291866 Plasma protease C1 inhibitor 20.6 7 50.85 68 3.3 0.009000  
IPI00022434 Putative uncharacterized protein ALB 33.17 15 84.91 83.54 7 0.009000  
IPI00877967 Putative uncharacterized protein F2 16.67 3 38.9 70.48 3.7 0.009000  
IPI00298828 Beta-2-glycoprotein 1 32.75 8 50.08 58.28 2.4 0.010000  
IPI00794184 cDNA FLJ37971 fis, clone CTONG2009958, highly similar to CERULOPLASMIN 5.2 2 43.15 54.38 2.2 0.010000  
IPI00910625 cDNA FLJ51265, moderately similar to Beta-2-glycoprotein 1 31.75 6 57.7 53.85 2.3 0.010000  
IPI00291262 Clusterin 21.83 8 64.87 81.71 4.9 0.010000  
IPI00019690 GRAM domain-containing protein 4 1.56 1 42.31 68.53 3.1 0.010000  
IPI00887739 similar to complement component C3, partial 3.01 2 99.17 85.98 6.6 0.010000  
IPI00556036 16 kDa protein 9.35 1 46.68 101.59 22.4 0.020000  
IPI00940791 20 kDa protein 26.49 4 68.87 66.04 2.5 0.020000  
IPI00022429 Alpha-1-acid glycoprotein 1 41.29 10 92.7 78.72 4 0.020000  
IPI00022395 Complement component C9 6.26 3 83.12 58.44 2.4 0.020000  
IPI00291867 Complement factor I 8.92 4 66.9 89.51 5.8 0.020000  
IPI00022371 Histidine-rich glycoprotein 18.48 6 52.49 65.69 2.7 0.020000  
IPI00016915 Insulin-like growth factor-binding protein 7 4.61 1 43.16 87.43 5.3 0.020000  
IPI00830047 Putative uncharacterized protein ENSP00000374858 (Fragment) 14.15 1 86.52 93.61 6.7 0.020000  
IPI00736860 Putative uncharacterized protein ENSP00000374988 (Fragment) 5.76 1 55.66 81.96 3.7 0.020000  
IPI00942787 42 kDa protein 34.82 13 61.83 91.49 4.4 0.030000  
IPI00922262 cDNA FLJ56822, highly similar to Alpha-2-HS-glycoprotein 6.94 2 82.08 90.01 4.1 0.030000  
IPI00641737 Haptoglobin 37.86 14 60.67 91.2 4.3 0.030000  
IPI00305461 Inter-alpha-trypsin inhibitor heavy chain H2 3.59 2 67.05 67.19 2.4 0.030000  
IPI00019038 Lysozyme C 35.14 3 99.3 100.08 6.8 0.030000  
IPI00855916 Transthyretin 55.43 12 56.66 51.02 1.9 0.030000  
IPI00910636 cDNA FLJ53848, highly similar to Inter-alpha-trypsin inhibitor heavy chain H2 2.76 1 105.83 80.27 3.2 0.040000  
IPI00032293 Cystatin-C 11.64 2 37.2 65.84 2.2 0.040000  
IPI00426060 Putative uncharacterized protein DKFZp686J11235 (Fragment) 24.11 8 50.51 70.47 2.4 0.040000  
IPI00218413 Biotinidase 3.13 1 152.06 59.58 2.5 0.050000  
IPI00296165 cDNA FLJ54471, highly similar to Complement C1r subcomponent 1.95 1 38.2 100.53 4.6 0.050000  
IPI00022418 Fibronectin 1.63 2 49.85 82.97 2.8 0.050000  
IPI00478493 Haptoglobin isoform 2 preproprotein 34.01 10 64.09 96.65 4.2 0.050000  
IPI00431645 HP protein 25.62 6 72.49 101.65 4.9 0.050000  
IPI00910432 cDNA FLJ57921, highly similar to Apolipoprotein D 10.94 1 32.12 115.55 6.7 0.060000  
IPI00156171 Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 4.06 2 154.37 118.59 9.6 0.060000  
IPI00026199 Glutathione peroxidase 3 29.2 6 62.58 52.9 1.9 0.060000  
IPI00292150 Latent-transforming growth factor beta-binding protein 2 1.04 1 35.63 129.41 22.1 0.060000  
IPI00002678 Opticin 14.46 2 44.87 57.55 1.8 0.060000  
IPI00852577 HCG2040025 33.02 2 79.85 92.66 3.2 0.070000  
IPI00021000 Osteopontin 10.19 2 73.71 115.05 6.2 0.070000  
IPI00514159 Inter-alpha (Globulin) inhibitor H2 8.02 1 61.82 59.15 1.8 0.090000  
IPI00029863 55 kDa protein (Alpha-2-antiplasmin precursor) 8.69 2 55.95 78.97 −1.8 0.100000  
IPI00002147 Chitinase-3-like protein 1 9.92 2 56.83 128.11 4.3 0.100000  
IPI00009650 Lipocalin-1 6.25 1 66.59 61.11 1.8 0.100000  
IPI00844156 SERPINC1 protein 3.86 1 77.33 80.74 2.3 0.100000  
IPI00383164 SNC66 protein 19.72 7 64.5 79.48 2.1 0.100000  
IPI00794403 23 kDa protein 17.82 3 80.99 159.25 9.8 0.200000  
IPI00922298 cDNA FLJ51445, highly similar to AMBP protein 7.04 1 64.26 81.95 2 0.200000  
IPI00022431 cDNA FLJ55606, highly similar to Alpha-2-HS-glycoprotein 13.39 4 49.95 144.5 5.1 0.200000  
IPI00029739 Complement factor H 1.46 1 48.36 116.78 2.6 0.200000  
IPI00021891 Fibrinogen gamma chain 7.51 2 86.57 136.01 3.5 0.200000  
IPI00292530 Inter-alpha-trypsin inhibitor heavy chain H1 2.09 1 66.21 40.03 −1.7 0.200000  
IPI00020986 Lumican 16.86 4 72.95 148.83 7.5 0.200000  
IPI00514285 Prostaglandin D2 synthase 21 kDa 14.73 2 62.05 70.38 1.7 0.200000  
IPI00658053 Putative uncharacterized protein CTSD 8.54 1 46.63 73.25 1.7 0.200000  
IPI00877925 Putative uncharacterized protein SERPINF2 6.44 1 74.76 66.92 −1.5 0.200000  
IPI00218732 Serum paraoxonase/arylesterase 1 6.2 1 41.72 55.4 1.5 0.200000  
IPI00006662 Apolipoprotein D 20.11 3 201.07 113.63 2.7 0.300000  
IPI00165972 Complement factor D preproprotein 15 2 63.48 59 1.5 0.300000  
IPI00930442 Putative uncharacterized protein DKFZp686M24218 5.67 1 99.15 71.25 1.7 0.300000  
IPI00935408 CFI protein 4.24 1 81.47 78.69 1.3 0.400000  
IPI00022417 Leucine-rich alpha-2-glycoprotein 29.97 5 59.22 42.95 1.2 0.400000  
IPI00334282 Protein FAM3C 7.05 1 38.27 61.04 −1.1 0.500000  
IPI00012503 Proactivator polypeptide 2.86 1 262.28 79.65 1.8 0.600000  
IPI00807428 Putative uncharacterized protein 22.98 3 168.41 135.85 2.1 0.600000  
IPI00011229 Cathepsin D 8.5 2 106.86 100.28 −1.5 0.700000  
IPI00423460 Putative uncharacterized protein DKFZp686G21220 (Fragment) 9.88 3 104.19 116.55 1.6 0.700000  
IPI00022337 Retinol-binding protein 3 5.93 3 71.53 69.31 1.1 0.800000  
IPI00301579 cDNA FLJ59142, highly similar to Epididymal secretory protein E1 7.96 1 39.81 61.76 1.2 0.900000  

Differentially expressed proteins detected using stringent filtering criteria are highlighted in bold.

Anshu, Mol Vis 2011; 17:1891-1900. http://www.molvis.org/molvis/v17/a206

©2011 Molecular Vision http://www.molvis.org/molvis/

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