Figure 8. Influence of ROCK inhibition on phosphorylation of FAK and Paxillin. A: The addition of 10% serum to serum-starved cells led to a significant increase in the tyrosine phosphorylation of FAK tyrosine at residue Tyr397 (middle lane). This increase was opposed by pretreatment with Y-39983 (1 μM; last lane). B: Similarly, the serum-induced phosphorylation of paxillin at Tyr118 (middle lane) was also opposed by Y-39983 (last lane).