Figure 1. Expression of myosin II, MYPT, ROCK, and MLCK isoforms in GTM3 cells. Expression pattern of genes in cultured cells was determined using total RNA- and sequence-specific oligonucleotide primers. A: These cells express two isoforms of myosin II, namely, MYH9 and MYH10. The other isoform, MYH14, was undetectable. In addition, the expression of both isoforms of MYPT, ROCK, and MLCK (B, C, D) was confirmed. Abbreviations: MYH9: myosin IIA; MYH10: myosin IIB; MYH14: myosin IIC; ROCK: Rho kinase; MYPT: myosin phosphatase; MLCK: myosin light chain kinase.