Figure 2. Effects of various treatments on calpain-induced lens proteolysis. A 12% SDS–PAGE analysis shows that proteolyzed proteins were seen at 19 kDa (lane 2–6, lane 9) while EDTA and calpain inhibitor E64 inhibited proteolysis. M, Fermentas prestained protein ladder^® marker; lane 1, normal; 2, control; 3, 100 μM PRX; 4, catalin equivalent to 100 μM PRX; 5, catalin equivalent to 80 μM PRX; 6, catalin equivalent to 32 μM PRX; 7, 3 mM EDTA; 8, 100 μM E64; 9, catalin-formulated vehicle only.