Figure 4. Multiple sequence alignments of PRSS56. A: Alignment of putative orthologs from multiple species, around locations of four familial missense variants believed to be pathogenic. Human sequence is top row of each subpanel, with mutated residue in larger font, with mutation in bold above human sequence. B: Predicted trypsin-like serine protease activity by NCBI Conserved Domains database with positions of mutations observed in our NNO families.