Figure 6. Expression of mRNAs for crystallin αA, EGF receptor (ERBB-1), TGFβ receptors, and EGF in primary cultured HLE cells (pHLE) and SRA01/04 cells (SRA). Total RNAs were extracted from primary cultured HLE and SRA01/04 cells, and were analyzed by RT–PCR using the primers listed in Table 1. RNA from HeLa cells was also analyzed as control. Aliquots (10 μl) of each RT–PCR product for crystallin αA (CRYAA; 100 ng, 35 cycles), EGF receptor (EGFR; 50 ng, 35 cycles), TGFβ receptor-1 (TGFBR1; 50 ng, 35 cycles), TGFβ receptor-2 (TGFBR2; 50 ng, 35 cycles), EGF (50 ng, 35 cycles) and β-actin (ACTB; 50 ng, 23 cycles) were electrophoresed on 2% agarose gels containing ethidium bromide.