Figure 6. Stability of αAG98R-crystallin. A: αAG98R Mutant protein was removed from −80 °C (stored for a year) and size exclusion chromatography was performed at room temperature after 0 – 12 days of incubation of the protein at 0.1 mg/ml at 37 °C. Day 0, Green; day 2, brown; day 6, blue and day 12, red. Inset picture shows enlarged view of the elution profile at the oligomer region, indicating loss of oligomer peak. B: SDS–PAGE of samples incubated for 0, 2, 6 and12 days. (Lane 1, 0; lane 2, 2 days; lane 3, 6 days; lane 4, 12 days).