Figure 2 of Saraswathy, Mol Vis 2011; 17:1814-1821.

Figure 2. Differential expression of mitochondrial proteins in early experimental autoimmune uveitis retina. On day 7 after immunization, mitochondria were isolated from retinas of B10.RIII mice induced with experimental autoimmune uveitis (EAU) and from control animals. The EAU and control samples were then differentially labeled with cyanine dyes (Cy3 and Cy5) and resolved using a single 2D gel. An internal standard containing equal amounts of each mitochondrial sample labeled with Cy2 was also used. The two images from both samples were overlaid, and the gel was scanned using a highly sensitive typhoon imager and processed by analysis software. The numbered spots were the differentially regulated proteins in EAU samples and these spots were picked for Mass Spectrometry analysis. The differentially expressed proteins were identified by MALDI-TOF/MS.