Figure 1. Purity of mitochondrial fractions from early experimental autoimmune uveitis retina. Mitochondria and cytosol were fractionated from the retinas of control (nonimmunized) and experimental autoimmune uveitis (EAU) mice. The purity of each fraction was tested using a mitochondrial marker, prohibitin, and two cytoplasmic markers, caspase 3 and calpain, by western blot analysis. There were no interorganelle contaminants.