Figure 6. Representative cross sectional
micrographs showing various retinal cell types transduced by
AAV2.CBA.eGFP after treatment with chondroitin ABC lyase. A-E:
A wide range of cell types were transduced in retinas treated with
AAV2.CBA.eGFP vector and 200 units of chondroitin ABC lyase. GFP
fluorescence was observed across the retinal layers, including cells
with the anatomic location and morphology of; A: ganglion
cells, B: Müller cells, C: INL cells, and D, E:
photoreceptors. FITC filter is shown in the left panel; DAPI filter is
shown in the right panel. Calibration bar 50 µm. Ganglion cell layer
(GCL), inner nuclear layer (INL), outer nuclear layer (ONL), outer
segments (OS). Green arrow shows a retinal ganglion cell. Blue arrow
shows a Müller cell. Orange arrow shows cells in inner nuclear layer.
Red arrow shows a photoreceptor cell.