Figure 1 of Zode, Mol Vis 2011; 17:1745-1758.


Figure 1. Immunohistochemical evaluation of transforming growth factor (TGF)-β2 expression in normal and glaucomatous optic nerve head (ONH) tissues. A representative immunostaining for TGF-β2 expression in age-matched normal (97 years) and glaucomatous (99 years) human ONH tissues. A: ONH tissue section from a normal human donor stained for TGF-β2 (red), glial fibrillary acidic protein (GFAP; green), and 4',6-diamidino-2-phenylindole (DAPI; blue) at lower magnification, and C: showing part of the lamina cribrosa (LC) in the white box, at higher magnification. B: ONH tissue section from glaucomatous human eyes stained for TGF-β2 (red), GFAP (green), and DAPI (blue) at lower magnification, and D: showing LC in the white box, at higher magnification. E: Control (no primary antibody for TGF-β2 and GFAP) merged with DAPI in normal ONH, and F: in glaucomatous ONH. G: Relative intensity measurements of TGF-β2 staining in the LC region in four age-matched normal and glaucomatous ONH tissues, showing significant higher staining for TGF-β2 in glaucomatous ONH tissues compared to normal human ONH tissues [student’s t-test, p<0.0013; ** versus normal human ONH tissues; n=4].