Molecular Vision: Garcia-Ayuso, Mol Vis 2011; 17:1716-1733. Table 2

Table 2. Quantification of the total population of RGCs in control and experimental retinas at different periods after light exposure.

	Control P60				Control P625
	FG		Brn3a		FG		Brn3a
Retina	Left	Right	Left	Right	Left	Right	Left	Right
1	79,090	82,653	78,732	87,628	80,535	86,019	85,923	80,348
2	77,567	74,237	90,197	93,896	82,306	83,783	82,264	86,695
3	76,965	79,478	87,835	81,566	77,599	76,522	73,943	84,896
4	82,944	84,076	90,263	82,157	82,363	75,536	85,813	84,025
5	86,150	86,657	83,680	90,021	87,124	86,927	82,157	82,043
Mean±SD	80,534±3,492	81,420±4,274	86,141±4,410	87,053±4,691	81,985±3,098	81,757±4,798	82,020±4,871	83,601±2,471
RE & LE Mean±SD	80,981±3,928		86,597±4,575		81,871±4,040		82,810±3,544
	0 h ALE		1 month ALE		3 months ALE		6 months ALE
	FG		FG		FG		FG
Retina	Left	Right	Left	Right	Left	Right	Left	Right
1	77,599	83,222	77,056	76,898	75,042	73,903	69,502	68,920
2	72,212	81,476	78,765	83,769	78,143	75,253	76,384	69,076
3	79,741	81,811	78,222	81,657	85,926	86,537	74,067	65,018
4	79,566	82,836	75,421	78,090	75,422	78,678	67,569	59,964
5	84,260	70,931	81,745	81,662	85,240	83,579	85,791	85,077
6	85,777	86,247	82,745	82,350	78,962	81,399	83,231	74,394
7	88,858	81,813			72,119	77,312	70,240	75,843
8	88,846	90,211
Mean±SD	82,107±5,470	82,318±5,120	78,992±2,543	80,737±2,423	78,693±4,822	79,523±4,219	75,255±6,487	71,184±7,545
RE & LE Mean±SD	82,212±5,298		79,865±2,633		79,108±4,549		73,219±7,324 §
	9 months ALE				12 months ALE
	FG		Brn3a		FG		Brn3a
Retina	Left	Right	Left	Right	Left	Right	Left	Right
1	79,691	74,467	#	#	76,939	81,085	#	#
2	79,849	77,367	#	#	73,287	72,567	#	#
3	63,045	67,771	#	#	49,278	58,418	#	#
4	50,506	49,366	#	#	70,271	61,707	#	#
5	79,281	73,755	#	#	70,240	64,704	#	#
6	64,954	66,632	#	#	64,199	71,006	67,899	69,783
7	71,375	73,308	72,185	73,010	66,378	70,817	64,725	67,955
8	71,112	71,992	73,506	72,970	54,433	70,131	67,779	70,999
9	70,420	69,746	73,952	69,119	71,656	63,259	61,138	76,592
10	65,203	73,296	69,397	73,944	70,404	70,004	69,647	69,997
Mean±SD	69,543±9,159	69,770±7,857	72,260±2,050	72,261±2,142	66,708±8,641	68,369±6,506	66,237±3,001	71,065±2,932
RE & LE Mean±SD	69,656±8,306§		72,260±1,941§		67,539±7,493§		68,651±2,413§

In all retinas, RGCs were identified by retrograde tracing with fluorogold (FG). In addition, RGCs present in control retinas and in some retinas analyzed 9 and 12 months ALE were identified as well by Brn3a immunodetection. The whole population of FG or Brn3a positive RGCs was automatically quantified. At the bottom of each group is shown the mean ± the standard deviation (SD) of RGCs counted in the left or right retinas. Because there was not a significant difference in the number of RGCs between right and left retinas within each group, is also shown the mean±SD of RGCs present in the right and left eyes of each group. P60 and P365 refer to control animals of 60 and 365 days of age, respectively. RE: right eye, LE: left eye. ALE: after light exposure. §: Statistically significant when compared to their respective control values (Brn3a or FG) (Mann–Whitney test p<0.001)