Figure 7 of Carr, Mol Vis 2011; 17:1701-1715.


Figure 7. A melanopsin (OPN4) peptide-blocking experiment was performed to test the specificity of the antibody for western blot. Due to the presence of multiple bands on the OPN4 western blot the OPN4 antibody was pre-incubated with a fivefold excess of 15 amino acid N-terminal blocking peptide in 10% milk/TBS-T for 2 h at room temperature before hybridization. Proteins extracted from fenretinide (FR) and dimethyl sulfoxide (DMSO) treated cells were incubated with OPN4 antibody pre-incubated with the blocking peptide (OPN4+BP) or OPN4 antibody alone. A single band of approximately 70 kDa specific to OPN4 was absent in the western blot containing the blocking peptide.