Figure 2. Immunohistochemical staining for Bcl-2, Bax, mutant p53, and TUNEL analysis positive cells in pterygium and normal conjunctiva samples. Human pterygium tissues (A, C, E, G); Human normal conjunctiva tissues (B, D, F, H). Bcl-2 showed strong expression in the cytoplasm throughout the entire width of the epithelial layer in pterygium (A). No expression was shown in the epithelial layer of Bcl-2 in normal conjunctiva (B). Bax showed strong expression in the cytoplasm throughout the entire width of the epithelial layer in both pterygium (C) and normal conjunctiva (D). mP53 immunostaining showed nuclear staining in the pterygium epithelial layer (E), but no expression in normal conjunctiva (F). For TUNEL analysis, the positive cells in pterygium showed nuclear staining in the basal layer of the epithelium (G). In normal conjunctiva, the positive cells showed nuclear staining in the whole width of the epithelial layer (H). All slides were counterstained with Mayer’s hematoxylin. Original magnifications: A-H, 400×.