Figure 2. Immunohistochemical analysis of expression patterns of K12, K13, and K19 in normal human histologic sections. Montage images of serial sections of the central cornea to the conjunctiva (A-C): Double staining of K12 (green) and K13 (red) showed the presence of K13 in the epithelia of the posterior limbus and conjunctiva (A). K19 expression was detected in the peripheral cornea, limbus, and conjunctiva (B). Double staining of K12 (green) and K19 (red) showed the overlapping expression of both cytokeratins (C). D: Expression of K12 (arrows) and K13 (arrowheads) were mutually exclusive. E: Details of the overlapping expression of K12 and K19. Arrowhead: cells that expressed both K12 and K19. Arrow: K12-expressing cell. Abbreviations: Conj., conjunctiva. Magnification bar in A, B, C represents 200 μm. Magnification bar in D and E represents 50 μm.