Table 2 of Piñeiro-Gallego, Mol Vis 2011; 17:1607-1617. Table 2 of Piñeiro-Gallego, Mol Vis 2011; 17:1607-1617.
Table 2. Oligonucleotides for genomic amplification and sequencing of BEST1 gene.
Exon |
Forward primer (5′-3′) |
Reverse primer (5′-3′) |
Tm (°C) |
Product size (bp) |
|---|---|---|---|---|
| 1 | AGCCCAACACCCTCCAAGAA | AAGGTGCCCCAGGTGGACT | 58 | 297 |
| 2 | CCCTACAAACCCCCAATCG | CTGGGAAGGATGTGGCTGG | 62 | 272 |
| 3 | AGTCTCAGCCATCTCCTCGC | CAGGCTCCAGACAGGCCA | 63 | 212 |
| 4 | AGAAAGCTGGAGGAGCCGA | AGGAATGGAAGATGGGTGGA | 61 | 413 |
| 5 | GCCATCCCTTCTGCAGGTT | GTACAGACAGGGCTGCCGC | 58 | 239 |
| 6 | GGGGCAGGTGGTGTTCAGA | CTAGGCTGGTGAGGCTGCC | 62 | 150 |
| 7 | TGATTTCAGGGTTCCCACCTAG | AGCTGCCTGAGACGAGGATG | 58 | 286 |
| 8 | AGGGTTTACAGAGCCTCACCTG | CATGGACCTTCCCCAAAGTG | 62 | 158 |
| 9 | CCTCCAAGTCATCAGGCACATA | CTAGTGCAGGGGTCTGCCTAG | 58 | 289 |
| 10A | ACTGGCTCAGCCCTGCATC | CAGGCTACTACAGTGCCCCAC | 62 | 340 |
| 10B | CCTTCAAGTCTGCCCCACTG | GAACCAGGGGCACTGCA | 62 | 417 |
| 11 | GGGACCTTCCATACTTATGCTG | TTCATCCCAAGACCT | 61 | 492 |