Table 1 of Audo, Mol Vis 2011; 17:1598-1606. Table 1 of Audo, Mol Vis 2011; 17:1598-1606.
Table 1. Oligonuleotide primer sequences used for PCR amplification of coding and flanking regions and direct sequencing.
| Amplified fragment | Primer sequenced (5′-3′) | Annealing temperature |
|---|---|---|
| Exon 1; 1st fragment | GGAACTCGCTGGAAGACTC | 58 °C |
| GTACAGTGGCTGGATCCTAG | 60 °C | |
| Exon 1; 2nd fragment | CTCTTGTGTCTCTCAGGAAC | 58 °C |
| CACCAGGTCGAAGACGTTG | 58 °C | |
| Exon 1; 3rd fragment | GGTTACTGTCTGCCAACGTG | 60 °C |
| AGACTCTCTCCAAACCACAG | 58 °C | |
| Exon 2 | CCTTCCTCTAACCTTTGTATC | 58 °C |
| GAGAATACGGTGTCTGAGAG | 58 °C | |
| Exon 3 | TCCCTCTAATGTTATCCTCTC | 58 °C |
| AAAGAGCTCCATGCACAGAG | 58 °C | |
| Exon 4 | CCAGGTGACTGTATTGTGTG | 58 °C |
| GTCTGGAGATTGGAGCGAG | 58 °C | |
| Exon 5 | GCCAACATCTAGAAAGATACC | 58 °C |
| CTGTGAGGAAGCAGAGAATAC | 60 °C | |
| TCAGCTCTAGAGCAGCTAAG | 58 °C | |
| Exon 6 | GGAAATGCTGAAGCCAGTTG | 58 °C |
| Exon 7 | CCTGTTCCACCTTCTAGTAG | 58 °C |
| GATTCGAACTCAGGCTGGTC | 60 °C | |
| Exon 8 | CTCAGCTTCTGTTTGCTCAC | 58 °C |
| GCTGTCATGGAGAGGAGAG | 58 °C | |
| Exon 9 | ACTCCTTCTCCGCCTGTTG | 58 °C |
| CTGGCCTTGCTGTACTCAC | 58 °C | |
| Exon 10 | TATATAAGCTGCTTCTCTCTTC | 58 °C |
| ACCTCCCATTCTGTGCCTG | 58 °C | |
| Exon 11 | GTGGTGTCCTTCCTAATACC | 58 °C |
| GTAGGGAGAGATGGCAAGTG | 60 °C | |
| Exon 12 | AAGCCAGGCCTGTCTAACC | 58 °C |
| GTCCTGCTCTCTTCCTCTC | 58 °C |