Figure 7. The effect of inflammatory cytokine on alternative pathway-related complement gene expression in BV-2 and retinal pigment epithelium cells. Primary RPE cells and BV-2 cells were treated with pro-inflammatory cytokines TNF-α (20 ng/ml), interferon (IFN)-γ (100 ng/ml), interleukin (IL)-6 (10 ng/ml), and lipopolysaccharides (LPS; 1 µg/ml) for 20 h. Cells were then collected for real-time RT–PCR (A) or conventional RT–PCR (B). Mouse liver RNA was used as a positive control in B. A: The effect of inflammatory cytokines on CFH and CFB genes expression in BV-2 cells. B: The effect of inflammatory cytokines on CFI and CFHR1 gene expression in BV-2 and RPE cells. n=3 in each group. *p<0.05 when compared to untreated control group using unpaired Student's t test. Experiments were repeated twice.