Figure 6. The effect of inflammatory cytokine on mannose binding lectin pathway-related complement gene expression in BV-2 and retinal pigment epithelium cells. Primary RPE cells and BV-2 cells were treated with pro-inflammatory cytokines tumor necrosis factor (TNF)-α (20 ng/ml), interferon (IFN)-γ (100 ng/ml), interleukin (IL)-6 (10 ng/ml), and lipopolysaccharides (LPS; 1 µg/ml) for 20 h. Cells were then collected for real-time RT–PCR (A) or conventional RT–PCR (B, C). Mouse liver RNA was used as a positive control in B and C. n=3 in each group. Experiments were repeated twice.