Figure 5. The effect of inflammatory cytokine on classical pathway-related complement gene expression in BV-2 and retinal pigment epithelium cells. BV-2 cells (A) and primary cultured mouse RPE cells (B, C) were treated with tumor necrosis factor (TNF)-α (20 ng/ml), interleukin (IL)-6 (10 ng/ml), interferon (IFN)-γ (100 ng/ml), or lipopolysaccharides (LPS; 1µg/ml) for 20 h. Cells were then collected for real-time RT–PCR (A, B) or conventional RT–PCR (C). Mouse liver RNA was used as a positive control in C. n=3 in each group. *p<0.05; **p<0.01; ***p<0.001 as compared to untreated control group using unpaired Student's t-test. Experiments were repeated twice.