Figure 3. Quantification of PEDF mRNA in ROP model at different postnatal day ages. Real time polymerase chain reaction values for PEDF expression in the retina of rat pups from selected postnatal days (P) 0 through P18 in RA or the ROP model. Increased PEDF expression associated with older developmental age (p=0.0001) or with the ROP model compared to RA (ANOVA, p=0.0185). *Post-hoc testing revealed increased PEDF mRNA at P11 in the ROP model compared to RA samples (p=0.0017). Error bars represent standard errors. Each time point had at least five retinas from different pups taken from at least two different litters. mRNA indicates mRNA.