Figure 2 of Hartmann, Mol Vis 2011; 17:1577-1587.


Figure 2. Tissue labeling of PEDF mRNA and protein in rat ROP model at P14. Sections of P14 rat retina in ROP model labeled by in situ hybridization for PEDF mRNA showing message in the ganglion cell layer (GCL), and in the inner and outer nuclear layers (INL, ONL; A, C). Control using sense probe showed no signal (B, D). Immunostaining for PEDF protein in P14 ROP model showed labeling in the inner and outer plexiform layers and inner and outer nuclear layers (E). No primary control showed nonspecific staining in the ganglion cell layer (F). Magnification calibration bars are 100 µm (A, B) and 50 µm (C-F).