Figure 8. Inhibition of protein kinase C (PKCδ) prevented ethambutol (EMB)-induced suppression of rod outer segments (ROS) uptake in ARPE 19. The fluorescein isothiocyanate (FITC)-labeled ROS were added to the cells without any treatment (CON), in the presence of 8 mM EMB alone, EMB plus 3 μM Rottlerin (EMB/rott), or Rottlerin alone (CON/rott) in ARPE19 for 5 h. The FITC-labeled ROS (green dot) uptake by the cells were observed under a phase contrast microscope (200× magnification). The nuclei were stained with PI (red) as counterstaining. The figure shown was representative of two repeated experiments.