Molecular Vision: Xu, Mol Vis 2011; 17:1537-1552. Table 1

Table 1. Primer information for the USH2A gene sequencing.

Primer	Forward sequence (5′-3′)	Reverse sequence (5′-3′)	Products (bp)	Tm (°C)
Exon 2	GCCTGGGATGAGCTTCAG	GGTTTGGAATTCAGGCTGA	840	62
Exon 3	CACCACTGTAACTGCACAATACC	CTGCTGCAGATTTTGTGAGTAGA	345	64
Exon 4	GTCTTCCCAGCTGAACAAAGTA	GTGGTAATTTGTTCAGTAGCCCTAG	382	60
Exon 5	GTCAGGTATTGCTTGGTAAACAG	CAGCATTTATCCTTTCGGTTC	173	62
Exon 6	CGAGTGACATTCATTTGTAACGA	GGCATTTGTTGCAATAACCA	437	58
Exon 7	TTTGAATCTAATAATTCCATGGTTTG	TGGTGGTGAAGGGAAGTCTC	372	58
Exon 8	CAACATTTTGATTTCTGTTTTGC	TGCTCTGACATCTTAATGTGCT	370	62
Exon 9	CACACAATGCATATAGTCCTAGG	TGTTAGGCCAAGATTAAGTTCAT	267	62
Exon 10	TGATATGTGCTTTACTTCTGGTG	GCATTGTAGATAGAAGCACACAG	356	62
Exon 11	TGGCAGGTAGAGATGAAAGG	GCAAATGCAGTCTTCAATTCTAC	371	62
Exon 12	CCCTGTCTTGTACCTAATGAGC	TTCCAGATGGTAATAGAGATGTGA	323	62
Exon 13	GCAGTAGCATTGTTTGTGTCTC	GTAGAAGCCACAAACCAGAAAC	816	58
Exon 14	GGGAATTAGTGCCTTGGTAGAG	GAAGTTATTGCTTTGCAACTGC	379	64
Exon 15	AAGCCGTCTTACTCTACAATGCT	TTCTGATGGGTTCTAAATGGAG	360	58
Exon 16	GCAATCCTGAATCAGAAAGACC	CCACAACAGCATTTATCCTCAA	354	64
Exon 17	GAGAGGAAAGCAGTTAGCAATG	GATTCTCATTCATGTCTTGACCA	626	64
Exon 18	AAGTAACCCCTTTGTCTGATGAGT	GGAAACATTTGCATTCAGAGG	378	62
Exon 19	TCAGAAACTAAATGAATGTGTGA	TGCCCTGTTTAATCAATATAGAG	379	60
Exon 20	TGGTGGTTGGCAATAATTCC	GAGTTAGTGAGGGAGGAGAAGACA	381	64
Exon 21	AGCCATACAGATACTTGAAACC	GCTATCAAAGGGCTGAATTAG	501	62
Exon 22	CCATGCGAGTATATTGCTGTG	GCTGAGGGCAAGTCACATTAC	451	62
Exon 23	CAGGAAAGCCAGAATGATGC	CCCAAAGGCAAATTCAACAG	447	58
Exon 24	CAGGCAATGAGGAGAGAGGA	CCTAAAGGAAATTTTTGGCACA	371	58
Exon 25	TGAATCATTAAGAGGCTTGCAG	TGTGTGCACCATTGGAATAAC	459	58
Exon 26	GGTCTTTCTGTCACTTCTGTGC	TTCAGTTATCAGGCGTGGTG	722	62
Exon 27	TGCTTTCAGGGAACTGTTTTG	GGTGCTGCTTTTAGCCTGAG	497	62
Exon 28,29	TGCTGCAGAGGACAAAAATG	GCTTCAGGGTAATAGTCCTTCC	579	62
Exon 30	TGCGGCCATTAAAAGTGAAG	TGCAGGCTTCCACTACTTTAG	434	58
Exon 31	GCAGAAAGGGAGGAAAATGAC	CAAATTAGGTTGGGGTTTGC	392	64
Exon 32	TGATTTATTGGTTTGGGTCTG	GCATTCTTGATTAAATTTGCAG	357	58
Exon 33	TGAAGCATCCTATATGTTATTGC	CCTCCCCTGATTGAACTCAC	392	58
Exon 34	ATTTCCTTTTTGCCCCTCAG	AGGATGGGAAGAGAGTTTTCAG	512	56
Exon 35	TTGGGGAAGTAAAGGTAGCAC	CCACAATCTCCCCAACTAGAG	431	62
Exon 36	AAATCACATCAAGAGTGCTTGC	CCTGCTTGAAAGGCTAGCTG	352	62
Exon 37	TGTGCTTTGATCCTGCTGAC	AACCAACATCTGTGGCTAAAAG	431	60
Exon 38	AATTGGCCAGGTCAACTCAG	TGTGAGGTGGATGAAAGCAG	614	64
Exon 39	CAGGAGTTCAGGAATGAAAATG	AAGTTTCAGTGGGAAGAAATCC	526	54
Exon 40	GAGATCTCATTTGATGGCAGAA	GGCTCATTTCTTTGCTTTGG	373	58
Exon 41	TGGCCTTTACCAAGTGTTCA	AAGGCCAAAACCCAGTTTCT	890	54
Exon 42	GCAAAATTCTAGGCCTCGTG	AAAGCCTCCTTCATTTCCCTAC	492	62
Exon 43	ATGCCACAGAACAGCCTGAG	AGCCGTGACAAAGGCAATAG	469	62
Exon 44	TTTTGTAGAGGGGTGGAAGG	TGTGTACATGGGGGAGGTTC	367	58
Exon 45	CATTTCCAAAACAAAGGCTCTC	TTAGCCTCCACCCCCTTC	464	58
Exon 46	TCATCATATCCCACTGGTCAC	CCCTTCTCTCTTTTCCCTTCC	599	54
Exon 47	AGGGAAGGTGGGATTCAGAC	TGTCATGGCTGAGAGGATACC	280	59
Exon 48	CCTCACGCCATGTGTTATTC	CCTTTCTTTTCCGTGGAGTC	530	54
Exon 49	TCGAATGATCCTGGAAAATACA	TTGTTGAGAGGGAGGTGTTTG	432	56
Exon 50	ACCTGTAAGTTGCCATGTGTG	TTTGGAGGACATGACCTTTTC	678	54
Exon 51	ATTTCAGCAACTGCCTGAGC	AAAGCTTCCCTGAGAACAGC	552	62
Exon 52	TGGGAAGCTGCAAAACTG	GGCCTCAAAGTATGATGGAATG	564	54
Exon 53	TCCGTATCCCTGCATCT	GGTTAGATGCATAGGGCAAT	500	56
Exon 54	ATTGCATTTCTTCCGAACAC	TCTCTCCTTCCAGCCATAGG	428	54
Exon 55	AAAGGGAAATGCTTCTCCAAG	CCCCCTAACCACAATGACAG	396	62
Exon 56	AGCCTCTTATGAGGTTCAGACC	CAAGCCTGAAGAATGGGAAC	422	54
Exon 57	GGGGATGGTGTGACTTTTG	ATGGCCAATGAATGAGGAAG	382	56
Exon 58	GGCAAAGAGTTTGCAATTTGTC	TTTATCCAGGAGACCGACTATG	399	62
Exon 59	CAAACATTTGTTGCCCATTC	GCCAGACTGTGATTTTTCTGG	488	54
Exon 60	TGCAAAAGGACAGGTTAAAA	GATTCTGCTGTGTTGGAGCA	343	54
Exon 61	TGACACCAGGAAGAAACAGC	TTATCCCCGTGACTACATTGC	638	54
Exon 62	TTTGGCCATGAGGTTCAGAG	TGAAGGGAGTTTTCCCACAG	417	60
Exon 63-A	AGGTTAAAAAGGGGCTAAGT	GGATATCACAGGTGGAGAGAGA	600	54
Exon 63-B	ATTCAGCTGGGCATACCTGT	CCATTGTCCAGGCAGATTTT	591	59
Exon 63-C	GAATGGAGGTTGCACAGCTA	GGCTCAGGCAATAGAAAGGTC	600	58
Exon 64	AATCTCGGCTCACTGCAAG	AGTGCCTTTTCAAATTGTGC	602	62
Exon 65	TGCTTTTGGTTGCCAATTTC	ACCGTAGAGCAACTGAGAACAG	440	58
Exon 66	TGTAGGAGGTGGGATCTTGG	CTGGGGAGTGCCAGGTAG	445	60
Exon 67	GAGCAGTTTCCTGCAAATGG	TCCCCACAAGAAAATCCTTC	579	56
Exon 68	GTTTGGATTGGTTCGGTTTG	CGTAAAGCTGGGGAACAGAG	344	60
Exon 69	CGTCATAACTTGCTTTGGAATC	CAACACTTGGCACAATTTCTTC	338	60
Exon 70	ATCAAATAGCAGGGCCAGAG	CCTCTCTTGGTCCCCACAC	462	60
Exon 71	GCTGCTAAATTCTGTAGGTGACA	TAAGTGCTCAGAGGCGAGTG	499	56
Exon 72	TGAGGCTTCTGAGGCTTAG	CTGCCAAACAGAACCAAGTG	651	58