Figure 4. Effects of increasing concentrations of advanced glycation end-products and LY294002 on the secretion of slit2 protein in human retinal pigment epithelium cells. Cells were treated with increasing concentrations of advanced glycation end-products (AGEs) ranging from 10 to 100 ug/ml and LY294002 for 24 h in serum-free media. Slit2 protein levels in human retinal pigment epithelium (RPE) cells were measured using an enzyme-linked immunosorbent assay, and it was demonstrated that AGEs resulted in an increase in slit2 secretion. LY294002 was shown to decrease the expression of slit2 protein under AGE treatment conditions (20 ug/ml). Values are reported as the mean±SD from three independent experiments. Asterisks denote values significantly different between the treated group and control group (p<0.05). Double asterisks denote values significantly different between the LY294002 (10 uM) group and the AGEs treatment (20 ug/ml) group (p<0.01).