Figure 10. Effects of recombinant N-SLIT2 protein on vascular endothelial growth factor protein levels in human retinal pigment epithelium cells. Vascular endothelial growth factor (VEGF) protein levels were measured by enzyme-linked immunosorbent assay, and it was determined that VEGF secretion from human retinal pigment epithelium (RPE) cells increased in a direct manner with increasing administration of recombinant N-SLIT2 protein (0.1, 1, 10, 100 ng/ml). Values are reported as the mean±SD from three independent experiments. Asterisks denote values significantly different between the treatment group and control group (p<0.05).