Table 1 of Sun, Mol Vis 2011; 17:1508-1513. Table 1 of Sun, Mol Vis 2011; 17:1508-1513.
Table 1. Primers used to amplify the coding and adjacent regions of the 6 genes.
Gene |
Primer name |
Primer sequence (5′→3′) |
Product length (bp) |
Annealing temperature (°C) |
|---|---|---|---|---|
| CRYAA | 1F | GCTGGGGGCGGGCACTTG | 552 | 68 |
| 1R | TGGGGACACAGGCTCTCG | |||
| 2F | GGTGACCGAAGCATCTCTGT | 295 | 68 | |
| 2R | CGTGACCCCCTTGTCCTC | |||
| 3F | ACCCGGCCCCTGTGAGAG | 438 | 59 | |
| 3R | AAAGGGAAGCAAAGGAAGACA | |||
| CRYGC | 1–2F | CCAAATAAAAGCAACACAGAGC | 671 | 63.8 |
| 1–2R | AAACCTCCCTCCCTGTAACC | |||
| 3F | CGCAGCAACCACAGTAATCT | 579 | 59.2 | |
| 3R | CCCACCCCATTCACTTCTTA | |||
| CRYGD | 1–2F | GGGCCCCTTTTGTGCGGTTCT | 643 | 65 |
| 1–2R | GTGGGGAGCAAACTCTATTGA | |||
| 3F | TGCTCGGTAATGAGGAGTTT | 506 | 63 | |
| 3R | AAATCAGTGCCAGGAACACA | |||
| GJA8 | 1aF | CAGATATTGACTCAGGGTTGC | 475 | 60 |
| 1aR | CCGCTGCTCTTCTTGACG | |||
| 1bF | ATTCGCCTCTGGGTGCTG | 571 | 58 | |
| 1bR | CCTTGGCTTTCTGGATGG | |||
| 1cF | GCAGCAAAGGCACTAAGAA | 578 | 60 | |
| 1cR | CACCTGAGCGTAGGAAGG | |||
| 1dF | ATCGTTTCCCACTATTTCC | 559 | 56 | |
| 1dR | GATCATGTTGGCACCTTTT | |||
| CRYBB1 | 1F | GGTAATGGAGGGTGGAAC | ||
| 1R | GAGAATAGGGACAGAGGATAAG | 672 | 62 | |
| 2F | GGAGGACAGGATCATTTCA | |||
| 2R | ATAATGTATGTGCCAGGAGTA | 387 | 62 | |
| 3F | CCTTTGGACTTTCCTACTG | |||
| 3R | GCTTTTGTGCTTATCATTT | 483 | 58 | |
| 4F | TAGACAGCAGTGGTCCCT | |||
| 4R | TTGATTACTCCTTCAACCC | 571 | 60 | |
| 5F | TAGCCAGGACAGAAGTGAGA | |||
| 5R | ATTGAACATGAAGAAGGGTT | 362 | 60 | |
| CRYBA4 | 1F | CCCTAGCCCAGTCACTCCT | ||
| 1R | TGAGCCTTGATTGCACCTCT | 289 | 60 | |
| 2F | GGCACCTGTGCTGTCTAGTG | |||
| 2R | GCCTAGGGAGAGGGGACCTA | 396 | 62 | |
| 3F | CTCCCCTAGTCGTGACAACC | |||
| 3R | TTTCAACTCTGGAACCTTTGA | 394 | 62 | |
| 4F | TTATTGCCCTTCCAAAAGGTT | |||
| 4R | TGTTCTCCTCTGGAATGTGG | 397 | 62 | |
| 5F | AAAAGAAAGGCTGGGATGGT | |||
| 5R | AAAACCGGTTCTTTGAAAAGATTA | 584 | 62 |