Table 1 of Sun, Mol Vis 2011; 17:1508-1513.
Table 1. Primers used to amplify the coding and adjacent regions of the 6 genes.
Gene |
Primer name |
Primer sequence (5′→3′) |
Product length (bp) |
Annealing temperature (°C) |
---|---|---|---|---|
CRYAA | 1F | GCTGGGGGCGGGCACTTG | 552 | 68 |
1R | TGGGGACACAGGCTCTCG | |||
2F | GGTGACCGAAGCATCTCTGT | 295 | 68 | |
2R | CGTGACCCCCTTGTCCTC | |||
3F | ACCCGGCCCCTGTGAGAG | 438 | 59 | |
3R | AAAGGGAAGCAAAGGAAGACA | |||
CRYGC | 1–2F | CCAAATAAAAGCAACACAGAGC | 671 | 63.8 |
1–2R | AAACCTCCCTCCCTGTAACC | |||
3F | CGCAGCAACCACAGTAATCT | 579 | 59.2 | |
3R | CCCACCCCATTCACTTCTTA | |||
CRYGD | 1–2F | GGGCCCCTTTTGTGCGGTTCT | 643 | 65 |
1–2R | GTGGGGAGCAAACTCTATTGA | |||
3F | TGCTCGGTAATGAGGAGTTT | 506 | 63 | |
3R | AAATCAGTGCCAGGAACACA | |||
GJA8 | 1aF | CAGATATTGACTCAGGGTTGC | 475 | 60 |
1aR | CCGCTGCTCTTCTTGACG | |||
1bF | ATTCGCCTCTGGGTGCTG | 571 | 58 | |
1bR | CCTTGGCTTTCTGGATGG | |||
1cF | GCAGCAAAGGCACTAAGAA | 578 | 60 | |
1cR | CACCTGAGCGTAGGAAGG | |||
1dF | ATCGTTTCCCACTATTTCC | 559 | 56 | |
1dR | GATCATGTTGGCACCTTTT | |||
CRYBB1 | 1F | GGTAATGGAGGGTGGAAC | ||
1R | GAGAATAGGGACAGAGGATAAG | 672 | 62 | |
2F | GGAGGACAGGATCATTTCA | |||
2R | ATAATGTATGTGCCAGGAGTA | 387 | 62 | |
3F | CCTTTGGACTTTCCTACTG | |||
3R | GCTTTTGTGCTTATCATTT | 483 | 58 | |
4F | TAGACAGCAGTGGTCCCT | |||
4R | TTGATTACTCCTTCAACCC | 571 | 60 | |
5F | TAGCCAGGACAGAAGTGAGA | |||
5R | ATTGAACATGAAGAAGGGTT | 362 | 60 | |
CRYBA4 | 1F | CCCTAGCCCAGTCACTCCT | ||
1R | TGAGCCTTGATTGCACCTCT | 289 | 60 | |
2F | GGCACCTGTGCTGTCTAGTG | |||
2R | GCCTAGGGAGAGGGGACCTA | 396 | 62 | |
3F | CTCCCCTAGTCGTGACAACC | |||
3R | TTTCAACTCTGGAACCTTTGA | 394 | 62 | |
4F | TTATTGCCCTTCCAAAAGGTT | |||
4R | TGTTCTCCTCTGGAATGTGG | 397 | 62 | |
5F | AAAAGAAAGGCTGGGATGGT | |||
5R | AAAACCGGTTCTTTGAAAAGATTA | 584 | 62 |