Figure 6. Representative western blots showing cytochrome c levels in gup mutants treated with diferuloylmethane. Cytochrome c (cyto c) levels were determined in embryos treated for 6 days. Mitochondrial and cytosolic fractions were normalized with cox IV or β-actin, respectively. Quantitation of western blot of cytochrome c localization in larvae is shown directly beneath western blots. Wildtype levels in each fraction are set to 1.0. Wt, wildtype; gup, untreated mutant larvae, gup+D, mutant plus diferuloylmethane.