Figure 5. Effect of diferuloylmethane on cell death in gup mutant larvae. A: Comparison of TUNEL-positive cells in coronal cryosections of the eye from mutant gup embryos (left), mutant gup embryos treated with 5 µM diferuloylmethane (center) and wildtype embryos (right) at 6 dpf. Histological sections counterstained with methyl green. B: Representative western blot of lysates from untreated mutant larvae (gup), mutant larvae treated with 5 µM diferuloylmethane (gup+D) and wildtype larvae (wt). Upper lanes, correspond to cleaved caspase-3; lower lanes, corresponding gapdh loading controls.