Figure 1. Effect of drug treatment on wildtype zebrafish. A: Wildtype embryos were dosed continuously from 10 hpf with diferuloylmethane. Percentage survival of embryos at 6 dpf was
determined for each treatment group (30 embryos/group), n=3, mean±SEM. Error bars smaller than the symbol are not visible.
B-E: Left panels, wholemount eye; center panels, coronal retinal section; right panels, wholemount larvae. B: Wildtype phenotype untreated at 6 dpf. C: Wildtype phenotype at 6 dpf treated with 5 µM diferuloylmethane from 10 hpf. D: Wildtype phenotype at 6 dpf following 300 µM zVAD-fmk treatment. E: Wildtype phenotype at 6 dpf following 300 µM zFA-fmk treatment. Scale bar left panel=200 µm. GCL, ganglion cell layer; INL,
inner nuclear layer; ONL, outer nuclear layer; RPE, retinal pigment epithelium, ON, optic nerve.