Figure 1. 2-DE gel of the lens proteins of 12-week-old Wistar rat stained with Coomassie brilliant blue R-250. Arrows indicate the unmodified proteins, and their assignment is shown, the modified crystallins are given the numbers only: spots 2–5,7,8 correspond to αA-crystallin, spots 10,11 – to βA4-crystallin, spots 15–20 – to βA3-crystallin, spot 22 – to βB1-crystallin, spot 23 – to βB3-crystallin, spots 26–27 – to βB2-crystallin, spot 28 – to αB-crystallin, spot 31 – to γE-crystallin and spot 33 – to γD-crystallin. The assignment of all indicated spots and the sequence coverage are also presented in Table 2.