Table 1 of Penha, Mol Vis 2011; 17:1436-1448. Table 1 of Penha, Mol Vis 2011; 17:1436-1448.
Table 1. Sequences of the specific primers used for real time-PCR amplification
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) | Amplicon size | NCBI accession |
|---|---|---|---|---|
| β-actin | CTGAACCCCAAAGCCAAC | CACCATCACCAGAGTCCATCAC | 147 bp | NM_205518 |
| HPRT | TGGCGATGATGAACAAGGT | GCTACAATGTGGTGTCCTCCC | 162 bp | NM_204848 |
| Insulin | CTTCTGGCTCTCCTTGTCTTTT | CAAGGGACTGCTCACTAGGGGC | 172 bp | NM_205222.2 |
| Insulin Receptor | CGCTGAGAATAACCCTGGTC | GCTGCCATCTGGATCATTTC | 60 bp | XM_001233398.1 |
| IGF-1 | CTTCAGTTCGTATGTGGAGACA | GATTTAGGTGGCTTTATTGGAG | 167 bp | NM_001004384.1 |
| IGF-1 Receptor | TCCAACACAACACTGAAGAATC | ACCATATTCCAGCTATTGGAGC | 167 bp | NM_205032.1 |
| Insulin (hydrolysis probe primers) | GGCTCTCTACCTGGTGTGTG | CTCGCTTGACTTTCTCGTATTCC | 149 bp | NM_205222.2 |
| Insulin-hydrolysis-probe | CACTCCTGCCTCGCCACGC |