Figure 7. Insulin receptor, insulin-like growth factor-1 receptor, and insulin-like growth factor–1 mRNA levels after 24 h of plus (+7D) and minus (−7D) lens treatment in the cartilaginous sclera (A) and fibrous sclera (B). Results are expressed as the mean normalized expression (MNE)±SEM. Nine animals were analyzed per group. Statistically significant differences between the treated groups and the control, as determined by one-way ANOVA (ANOVA) are denoted in the graph (* for p<0.05 and ** for p<0.01). In the cartilaginous sclera, the mRNA contents of the two receptors were not significantly different. In the fibrous sclera, the expression of the insulin and the insulin-like growth factor (IGF)-1 receptor (IGF-1R) was higher in the minus lens–treated group compared to controls.