Figure 6. Insulin receptor, insulin-like growth factor–1 receptor, and insulin-like growth factor–1 mRNA levels after 4 and 24 h of plus (+7D) and minus (−7D) lens treatment in the choroid. Results are expressed as the mean normalized expression (MNE)±SEM. For the 4 h experiment, six animals per groups were used; nine per group were used for the 24 h experiment. Statistically significant differences between the treated groups and the control, as determined by one-way ANOVA (ANOVA) are denoted in the graph (* for p<0.05 and ** for p<0.01). mRNA levels for insulin receptor (IR) were significantly increased after 4 h and 24 h of minus lens treatment, and the insulin-like growth factor (IGF)-1 receptor (IGF-1R) mRNA level was higher in the minus lens–treated group compared to the plus lens–treated group after 24 h of lens wear.