Table 1 of Kumar, Mol Vis 2011; 17:1414-1419. Table 1 of Kumar, Mol Vis 2011; 17:1414-1419.
Table 1. Primers used for PAX6 amplification.
| Sample number |
Forward primer sequence (5′-3′) |
Reverse primer sequence (5′-3′) |
Product size (bp) |
Annealing temp (°C) |
|---|---|---|---|---|
| 1 | GGAGTTCAGGCCTACCTGATGCAG | GAGAAGAGCCAAGCAAACGCCCTC | 313 | 55 |
| 2 | CGCCGAGGTTGGCACAGGTT | AGCATGGGCTGGGGAGAGCA | 710 | 55 |
| 3 | CGTTTTGATGCATCTTCAGGCAGTG | GGGCATTCCTCTCTGTTCCCCCA | 703 | 62 |
| 4 | TGGTGAAGGACCCCCTCCGC | ACACACACGCACCCACCAGC | 647 | 61 |
| 5 | ACCAGGCCCCTTTTGGAGGCT | TGGCATTCAGTGACCTTTCTGTGGC | 554 | 62 |
| 6 | GGAGTGGGGAGGTGGGAACCA | AGGCCCTGAGCCACTCCTCAC | 837 | 60 |
| 7 | AGCTGTGGCCAGTGGAAGGAC | TGGGCCCCCTACTGAGCTTCG | 636 | 62 |
| 8 | TCCTTTGGATTGGGGTGGGGG | CGTGGCAAAGCTTGTTGATCATGG | 713 | 62 |