Table 2 of Han, Mol Vis 2011; 17:1381-1396.

Table 2. Primers used in semiquantitative reverse-transcription (RT)-PCR of xlProminin homologs.

Targets Primer pairs Sequences (5′-3′) Expected MWs
xlProminin-1 1 GAAATTGGCTTTATCATTGCGGCAGTA 1939-1993a bps
    AGTAGCCAATGACTTCATCCATTAACTT  
  2 AACCTTACCAACCAGATTAGAGGATCA 1601-1655a bps
    ACCTTCCCCTCGATATCAAAGGATG  
xlProminin-2 1 CAGAAGGTCCAAGAGGAGTTTGC 1800 bps
    CCAGGGATTAGTTATATTGTCACACA  
  2 GTGCTTGAAAAGATTGTATCTTCTTTC 1370 bps
    AAGGCATTGGCTTCACTCTGTAGC  
xlProminin-3 1 GAAGTTGACTTAATAATTGGCCAAAGT 1881 or 1902b bps
    GAGAGCAATGAGAGAGACATTTCAG  
  2 CATGTTGGCTGGAAATATCTGCATGTT 1436 bps
    AACTGTCTTTCGCCAGCGTAGC  
X. laevis β-actin (as control)   GGCTATGCTCTACCACATGCCA 532 bps
    TGGAGCCACCAATCCAGACA  

aThe expected MWs of these fragments varies with the presence or absence of the alternatively spliced exons 8a (24 bps), 11a (12 bps) and 19 (18 bps). The differences in sizes of these PCR products are too small to be resolved on a 1% agarose gel.

bThe expected MWs of these fragments varies with the presence or absence of the alternatively spliced exons 24a (21 bps). The differences in sizes of the two PCR products are too small to be resolved on a 1% agarose gel.

Han, Mol Vis 2011; 17:1381-1396. http://www.molvis.org/molvis/v17/a155

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