Table 1 of Han, Mol Vis 2011; 17:1381-1396. Table 1 of Han, Mol Vis 2011; 17:1381-1396.
Table 1. Primers used in cloning of xlProminin-1, 2 and 3 cDNAs.
| Targets | Sequences (5′-3′) | Purpose |
|---|---|---|
| xlProminin-1 | CGGGGTACCGTAGATCTCTTGGCATTCGCTAATG | RT–PCR |
| CGCGGATCCTTATGATAACCATTATTACCATTTTCCA | RT–PCR | |
| ATTGACTGCTCCAGTGGCAC | 5′RACE outer primer | |
| CATTAGCGAATGCCAAGAGATCTAC | 5′RACE inner primer | |
| GTTTGGTTTGGGAGGAGCAACTGTG | 3′RACE outer primer | |
| ATGGACACCGAAGATGTTTATGATG | 3′RACE inner primer | |
| xlProminin-2 | CAGAAGGTCCAAGAGGAGTTTGC | RT–PCR |
| CCAGGGATTAGTTATATTGTCACACA | RT–PCR | |
| ATTTGCATCGACTACAGCCTGC | 5′RACE outer primer | |
| TTTTCAAGCACCAGGGGAAGGC | 5′RACE inner primer | |
| TTGGAAAACCTGGCAACCGTGC | 3′RACE outer primer | |
| AACTCCTTGGCTGAACTGGTCC | 3′RACE inner primer | |
| xlProminin-3 | CATGTTGGCTGGAAATATCTGCATGTT | RT–PCR |
| TTGCCGAGATGTTGTCATCTCCAT | RT–PCR | |
| TAGTAGCCACCAGGAGCTTAGC | 5′RACE outer primer | |
| TCACGGCATTTACGGTGTTCAC | 5′RACE inner primer | |
| AATTTTGCTCGCTGTGGACCAGTTGCCAGA | 3′RACE outer primer | |
| TGCAAAGCACTACAGGAGGATGAAGACTTCT | 3′RACE inner primer | |
| Adaptor or anchor primers for RACE | GGCCGCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG | 5′RACE adaptor primer |
| GGCCGCACGCGTCGACTAGTAC | 5′RACE anchor primer | |
| CGACTCACTATAGGGCGATTTTTTTTTTTTTTTTT | 3′RACE adaptor primer | |
| CGGGGTACCGTAATACGACTCACTATAGGGCGA | 3′RACE anchor primer |