Table 1 of Ding, Mol Vis 2011; 17:1343-1349. Table 1 of Ding, Mol Vis 2011; 17:1343-1349.
Table 1. Oligonucleotide primers used for PCR.
Exon |
Forward |
Reverse |
Annealing temperature (°C) |
Product length (bp) |
|---|---|---|---|---|
| CRYAA-1 | 5′-AGCAGCCTTCTTCATGAGC-3′ | 5′-CAAGACCAGAGTCCATCG-3′ | 61.4 | 584 |
| CRYAA-2 | 5′-GGCAGGTGACCGAAGCATC-3′ | 5′-GAAGGCATGGTGCAGGTG-3′ | 61.4 | 550 |
| CRYAA-3 | 5′-GCAGCTTCTCTGGCATGG-3′ | 5′-GGGAAGCAAAGGAAGACAGA-3′ | 61.4 | 511 |
| CRYAB-1 | 5‘-AACCCCTGACATCACCATTC-3′ | 5′-AAGGACTCTCCCGTCCTAGC-3′ | 64.7 | 250 |
| CRYAB-2 | 5′-CCATCCCATTCCCTTACCTT-3′ | 5′-GCCTCCAAAGCTGATAGCAC-3′ | 62 | 350 |
| CRYAB-3 | 5′-TCTCTCTGCCTCTTTCCTCA-3′ | 5′-CCTTGGAGCCCTCTAAATCA-3′ | 62 | 400 |
| CRYGC-1 | 5′-TGCATAAAATCCCCTTACCG-3′ | 5′-CCTCCCTGTAACCCACATTG-3′ | 59 | 514 |
| CRYGC-2 | 5′-TGGTTGGACAAATTCTGGAAG-3′ | 5′-CCCACCCCATTCACTTCTTA-3′ | 59 | 430 |
| CRYGD-1 | 5′-CAGCAGCCCTCCTGCTAT-3′ | 5′-GGGTCCTGACTTGAGGATGT-3′ | 61.4 | 550 |
| CRYGD-2 | 5′-GCTTTTCTTCTCTTTTTATTTCTGG-3′ | 5′-AAGAAAGACACAAGCAAATCAGT-3′ | 61.4 | 308 |
| CRYGS-2 | 5′-GAAACCATCAATAGCGTCTAAATG-3′ | 5′-TGAAAAGCGGGTAGGCTAAA-3′ | 61.4 | 575 |
| CRYGS-3 | 5′-AATTAAGCCACCCAGCTCCT-3′ | 5′-GGGAGTACACAGTCCCCAGA-3′ | 61.4 | 479 |
| CRYBA1–1 | 5′-GGCAGAGGGAGAGCAGAGTG-3′ | 5′-CACTAGGCAGGAGAACTGGG-3′ | 62 | 550 |
| CRYBA1–2 | 5′-AGTGAGCAGCAGAGCCAGAA-3′ | 5′-GGTCAGTCACTGCCTTATGG-3′ | 61.4 | 508 |
| CRYBA1–3 | 5′-AAGCACAGAGTCAGACTGAAGT-3′ | 5′-CCCCTGTCTGAAGGGACCTG-3′ | 62 | 463 |
| CRYBA1–4 | 5′-GTACAGCTCTACTGGGATTG-3′ | 5′-ACTGATGATAAATAGCATGAACG-3′ | 62 | 355 |
| CRYBA1–5 | 5′-GAATGATAGCCATAGCACTAG-3′ | 5′-TACCGATACGTATGAAATCTGA-3′ | 62 | 597 |
| CRYBA1–6 | 5′-CATCTCATACCATTGTGTTGAG-3′ | 5′-CATCTCATACCATTGTGTTGAG-3′ | 62 | 528 |
| CRYBB2–1 | 5′-GTTTGGGGCCAGAGGGGAGTGGT-3′ | 5′-TGGGCTGGGGAGGGACTTTCAGTA-3′ | 59 | 350 |
| CRYBB2–2 | 5′-CCTTCAGCATCCTTTGGGTTCTCT-3′ | 5′-GCAGTTCTAAAAGCTTCATCAGTC-3′ | 59 | 330 |
| CRYBB2–3 | 5′-GTAGCCAGGATTCTGCCATAGGAA-3′ | 5′-GTGCCCTCTGGAGCATTTCATAGT-3′ | 59 | 360 |
| CRYBB2–4 | 5′-GGCCCCCTCACCCATACTCA-3′ | 5′-CTTCCCTCCTGCCTCAACCTAATC-3′ | 60.7 | 230 |
| CRYBB2–5 | 5′-CTTACCCTTGGGAAGTGGCAATGG-3′ | 5′-TCAAAGACCCACAGCAGACAAGTT-3′ | 60.7 | 600 |
| GJA3–1 | 5′-CGGTGTTCATGAGCATTTTC-3′ | 5′-CTCTTCAGCTGCTCCTCCTC-3′ | 61.4 | 450 |
| GJA3–2 | 5′-GAGGAGGAGCAGCTGAAGAG-3′ | 5′-AGCGGTGTGCGCATAGTAG-3′ | 61.4 | 450 |
| GJA3–3 | 5′-TCGGGTTCCCACCCTACTAT-3′ | 5′-TATCTGCTGGTGGGAAGTGC-3′ | 61.4 | 300 |
| GJA8–1 | 5′-CCGCGTTAGCAAAAACAGAT-3′ | 5′-CCTCCATGCGGACGTAGT-3′ | 61.4 | 420 |
| GJA8–2 | 5′-GCAGATCATCTTCGTCTCCA-3′ | 5′-GGCCACAGACAACATGAACA-3′ | 61.4 | 330 |
| GJA8–3 | 5′-CCACGGAGAAAACCATCTTC-3′ | 5′-GAGCGTAGGAAGGCAGTGTC-3′ | 61.4 | 350 |
| GJA8–4 | 5′-TCGAGGAGAAGATCAGCACA-3′ | 5′-GGCTGCTGGCTTTGCTTAG-3′ | 61.4 | 500 |