Table 1 of Aggarwal, Mol Vis 2011; 17:1305-1309.

Table 1. Depicting the primers used in the present study for amplification of PAX6.



Exon

5'→3'

mer
Ta
(°C)		 PCR
product
size (bp)
1F GTTTGAAAAGGGAACCGTGG 20 61 243
1R CGGCTGGAGAGTGAGAGATA 20    
2F GCATAGGTGTGCTGGCTGCAG 21 62 350
2R GGAGACCTGTCTGAATATTGC 21    
3F TCAGAGAGCCCATGGACGTA 20 61 193
3R CTGTTTGTGGGTTTTGAGCC 20    
4F CCAGGTCCACCTCGGTTGGGA 21 67 208
4R TGTGTCCTCCCCTGACCCTC 20    
5F CCTCTTCACTCTGCTCTCTT 20 60 288
5R CATAATTAGCATCGTTTACAGTAA 24    
5AF TGAAAGTATCATCATATTTGTAG 23 57 236
5AR GGGAAGTGGACAGAAAACCACA 22    
6F GTGGTTTTCTGTCCACTTCC 20 60 299
6R AGGAGAGAGCATTGGGCTTA 20    
7F CAGGAGACACTACCATTTGG 20 60 252
7R ATGCACATATGGAGAGCTGC 20    
8F GGGAATGTTTTGGTGAGGCT 20 60 371
8R CAAAGGGCCCTGGCTAAATT 20    
9F ACAGTTTGGTCAACATATTTTG 22 60 217
9R CAAGCACCTCTGTCTCTAGG 20    
10F GTAGACACAGTGCTAACCTG 20 60 243
10R CCCGGAGCAAACAGGTTTAA 20    
11F TTAAACCTGTTTGCTCCGGG 20 63 240
11R CCCTGAGCCACTCCTCAC 18    
12F GCTGTGTGATGTGTTCCTCA 20 63 228
12R TGCAGCCTGCAGAAAGCAGTG 21    
13F CATGTCTGTTTCTCAAAGGGA 21 61 228
13R TCCTGAAAGCTCAACTGTTGTG 22    

PCR conditions: 95 °C×7 min, 95 °C×30 s, Ta °C×30 s, 72 °C×30 s (45 cycles of step 2,3,4), 72 °C×10 min. MgCl2 concentration (mM) for PCR reaction of each exon: mM MgCl2: exon 2; 1.5 mM MgCl2: exon 1, 3, 4, 5FR, 6, 7, 8, 9, 10, 11, 12, 13; 2.0 mM MgCl2: exon 5AFAR.

Aggarwal, Mol Vis 2011; 17:1305-1309. http://www.molvis.org/molvis/v17/a146

©2011 Molecular Vision http://www.molvis.org/molvis/

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