Figure 6. Localized expression of Glb1l3 in retinal cell layers. Wild-type retina sections were used to perform combined in situ hybridization (ISH) and immunohistochemistry (IHC) using cell type-specific retinal markers of the rods (recoverin), the cones (cone arrestin; cArr), the horizontal (calbindin) and bipolar (PKCα) cells of the INL, the Müller cells (GFAP), and the ganglion cells (Brn3b) of the GCL. As the staining for Brn3b was too weak to be detected in a combined experiment, two adjacent retina sections were used for Glb1l3 in situ hybridization and Brn3b immunohistochemistry. Glb1l3 mRNA was mainly expressed in the rods, in the cells of the INL—including horizontal and bipolar cells, and in the ganglion cells. OS, outer segments; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer.