Figure 1 of Le Carré, Mol Vis 2011; 17:1287-1297.


Figure 1. Transcriptional alteration of β-galactosidase (Glb)-related members in Rpe65−/− retinas. A: mRNA expression from wild-type (wt, black diamonds) and Rpe65−/− retinas (gray squares) was assessed at 2, 4 and 6 months of age (2–6 m) by oligonucleotide microarray. B: Retinal mRNA expression was validated by real-time PCR. Relative mRNA levels were expressed as fold inductions relative to samples from 2-month-old WT mice arbitrarily set to 1. Downregulated mRNA expression in diseased retinas at all ages was specific to Glb1l3 (p<0.001 by two-way ANOVA (ANOVA) for Rpe65−/− versus WT), and to a less extent to Glb1l2 (p<0.05 by two-way ANOVA for Rpe65−/− versus WT), while expression of the other Glb-related members remained unaltered. Data are the mean±standard error of the mean (SEM) of 3 (A) and 4 (B) independent animals. ND: not described; the Glb1l2 gene was not present in the Affymetrix Mouse Genome 430.2 Genechip.