Figure 4. Cellular Localization of Snail in ARPE-19 cells. A: Cellular localization of Snail (red) at 2 days after passage. Snail is observed in both the nuclei and cytoplasm. Nuclei were counterstained with Hoechst 33258 (blue). B: Cellular localization of Snail (red) at 7 days after passage. Snail expression is decreased and observed mainly in the cytoplasm, but not in the nuclei. C: Negative control. Scale bar=50 µm.