Figure 2. Real time PCR confirmation of selected genes affected by PITX2 siRNAs. All genes tested were among PITX2-filtered genes except DKK1 and KCNJ2. For each TM, the four pair of bars show log₂ fold change in expression based on data of each of two PITX2 siRNAs compared to each of two controls exposed to scrambled siRNA. Standard deviations for real time PCR data are shown. See text for further details on real time PCR experiments.