Figure 3. GLC cells show elevated cytosolic Ca²⁺. Flow cytometry was performed in Fluo-4/AM loaded NLC and GLC cultures. LC cells were contained in 1 ml isotonic Ca²⁺ free solution and the fluorescence intensity was measured. Addition of 2 mM extracellular Ca²⁺ induced a rise of cytosolic Ca²⁺ in both NLC and GLC and this response was is more intense and has greater variability in the GLC cells than in normal control LC cells. Results shown are tracings of a typical experiment with similar results observed in 3 separate experiments.