Figure 7. FK506 binding protein 51 overexpression protects 661w cell culture from staurosporine-induced caspase 3 activation. Staurosporine treatments for 6 h significantly increased caspase 3 activation, a marker of apoptosis, in 661w cell culture. FKBP51 overexpression in 661w cell cultures significantly decreased caspase 3 activation during staurosporine treatments. Caspase 3 activity was assayed using a colorimetric caspase 3 detection kit. Intensity was measured using Image J. FKBP51 significantly protected against caspase 3 activation during staurosporine treatment at 10 nM (p=0.006, n=3), 100 nM (p=0.001, n=3), and 1 μM (p<0.001). Significance was obtained through ANOVA (ANOVA) and the Mann–Whitney test. Error bars represent SEM.