Figure 3. FK506 (tacrolimus) binding
protein 51 overexpression protects 661w cell culture from
staurosporine. A: The FK506 Binding Protein 51 (FKBP51) protein
significantly protected the 661w neuronal cell culture from
staurosporine-induced apoptosis. Cell survival was monitored with a
calcein-AM/propidium iodide cell-survival assay. The 661w neuronal cell
cultures were stably transfected with either an FKBP51 expression
vector or parental empty vector. Cells were treated with or without
varying concentrations of staurosporine (10 nM, 100 nM, and 1 µM) for
24 h (scale bar=100 µm). B: FKBP51 overexpression significantly
protected the 661w neuronal cell culture from staurosporine-induced
apoptosis at 100 nM (p=0.013) and 1 μM (p≤0.001) concentrations (n=3).
Error bars represent SEM.