Table 1 of Yang, Mol Vis 2011; 17:1128-1135. Table 1 of Yang, Mol Vis 2011; 17:1128-1135.
Table 1. Primers used for PCR amplification and sequencing of TSPAN12.
Exon |
Forward primer (5′-3′) |
Reverve primer (5′-3′) |
Amplicon size (bp) |
Annealing tempeture (°C) |
|---|---|---|---|---|
| 2 | GCTGTGGGAAGCGTGAT | TTAGCCATGCCCTTTGG | 281 | 58 |
| 3 | TTCAAGATGCAGCAAATGGTAAT | GCTATGGGCAGGAAAAACTT | 329 | 58 |
| 4 | ATGTCTTGGGTGCATTT | AAGCGTCCCTTCTTACA | 321 | 56 |
| 5 | AGGGCTCACTGAGATGA | TCTTGGGCAGTTCTTTC | 382 | 55 |
| 6 | TTAGAAGACATTCCGAGTA | AATTGCACCAGAGGTTA | 360 | 55 |
| 7 | TCCTTTACTACATTTCTATC | TAGCTTTCTTCTGCTTC | 462 | 52 |
| 8 | ACAGATTGTTTGCTTTC | AGGTGTTATTTTATGGC | 505 | 52 |